Determination of genome size variation among varieties of Ilex cornuta (Aquifoliaceae) by fow cytometry

Ilex cornuta Lindl. & Paxton is a commercially important horticultural species worldwide, and extensive cultivation hybridization have produced many varieties. Despite the considerable breeding, selection, widespread domestication, which may significant role in composition of genomes, there are no other previous reports intraspecific genome size variation different cultivars or hybrids this species. In present work, 12 varieties I. was assessed analyzed through high-resolution flow cytometry (FCM). Nuclear DNA using nuclei isolated from young leaves, used propidium iodide (PI) staining, with rice (Oryza sativa cv. Nipponbare) as internal reference. As result, statistically differences were detected among all diploid considered. The estimated (2C value) ranged 1.47 to 1.80 pg, 1.22-fold an average 1.65 pg. domestication interspecific induced cornuta, exhibited wider range compared that cultivars. summary, useful tool analyze cornuta. first report sizes would provide data for further research on enrich C value database L. What’s more, our findings could be foundation future sequencing breeding programs.